Editors' ChoiceMicrobial Genetics

Analyzing the Staph

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Science's STKE  25 Sep 2001:
Vol. 2001, Issue 101, pp. tw351
DOI: 10.1126/stke.2001.101.tw351

Staphylococcus aureus is a common cause of infection for which there are diminishing therapeutic options. By combining two existing technologies in a new way, Ji et al. developed a library of antisense RNAs that are inducible with tetracycline, which means the respective genes can be turned on and off not just in vitro but also in infected mice. The roles played by specific genes in bacterial growth and behavior can be examined in complex ecologies, such as at the site of infection, and thereby, clinically relevant drug or vaccine targets can be identified.

Y. Ji, B. Zhang, S. F. Van Horn, P. Warren, G. Woodnutt, M. K. R. Burnham, M. Rosenberg, Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science 293, 2266-2269 (2001). [Abstract] [Full Text]

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