Protocol

Analysis of Protein Arginine Methylation and Protein Arginine-Methyltransferase Activity

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Science's STKE  31 Jul 2001:
Vol. 2001, Issue 93, pp. pl1
DOI: 10.1126/stke.2001.93.pl1

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  • Improved PRMT Substrate Detection

    In "Analysis of Protein Arginine Methylation and Protein Arginine Methyltransferase Activity" by Mowen and David, the authors outline procedures for the purification and use of recombinant arginine methyltransferases from bacteria. The methyltransferase activity assay supplied here relies on the PRMT-dependent incorporation of tritiated S-adenosyl-L-methionine (3H-SAM) into a protein or peptide substrate. The lab...

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    Competing Interests: None declared.

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