Candid Camera on Secretion

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Science's STKE  09 Nov 2004:
Vol. 2004, Issue 258, pp. tw409
DOI: 10.1126/stke.2582004tw409


The interaction of SNARE proteins, like SNAP25, syntaxin, or synaptobrevin, is a key event in the fusion intracellular membranes, such as when secretory vesicles fuse with the plasma membrane during regulated secretion. An et al. engineered a series of fluorescent SNAP25 analogs that change color when SNAREs interact in living cells. In time-resolved recordings, SNARE complexes could be observed to assemble and disassemble when single cells were stimulated to secrete.

S. J. An, W. Almers, Tracking SNARE complex formation in live endocrine cells. Science 306, 1042-1046 (2004). [Abstract] [Full Text]

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