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Science's STKE  23 Nov 2004:
Vol. 2004, Issue 260, pp. tw426
DOI: 10.1126/stke.2602004tw426

Existing fluorescent protein highlighting techniques are irreversible and preclude repeated monitoring of the same protein to study its temporal regulation. Within cells, protein movement is regulated by many different factors and may be altered by changes in the cellular state. Measurements of protein dynamics are affected by the geometry of both the cells and the highlighted regions, and any changes in movement should ideally be assessed with data from a single cell. Ando et al. describe the engineering and application of a fluorescent protein, Dronpa, that can be reversibly highlighted to study spatiotemporal protein dynamics in living cells. The authors directly visualized the influx and efflux of a key regulator of intracellular signaling, mitogen-activated protein kinase, into and out of the nucleus.

R. Ando, H. Mizuno, A. Miyawaki, Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306, 1370-1373 (2004). [Abstract] [Full Text]

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