Editors' ChoiceCell Biology

Life Without Dynamin

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Science's STKE  01 May 2007:
Vol. 2007, Issue 384, pp. tw155
DOI: 10.1126/stke.3842007tw155

Dynamin 1 is a neuron-specific guanosine triphosphatase involved in the endocytic recycling of synaptic vesicle membranes. Ferguson et al. created genetically engineered mice lacking dynamin 1 and found, surprisingly, that they contained functional synapses and had limited postnatal viability. However, the synapses of these dynamin 1 knockouts contained branched, tubular plasma membrane invaginations capped by clathrin-coated pits, consistent with dynamin 1’s proposed role in clathrin-coated vesicle scission. Also, after strong stimulation, synaptic vesicle endocytosis was severely impaired but could resume efficiently upon stimulus termination. This finding reveals the existence of a dynamin 1-independent mechanism that can support limited synaptic vesicle endocytosis.

S. M. Ferguson, G. Brasnjo, M. Hayashi, M. Wölfel, C. Collesi, S. Giovedi, A. Raimondi, L.-W. Gong, P. Ariel, S. Paradise, E. O’Toole, R. Flavell, O. Cremona, G. Miesenböck, T. A. Ryan, P. De Camilli, A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Science 316, 570-574 (2007). [Abstract] [Full Text]

P. J. Robinson, How to fill a synapse. Science 316, 551-553 (2007). [Summary] [Full Text]

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