Contents
Vol 2007, Issue 388
Contents
Editorial Guide
- Focus Issue: Single Molecules—Zooming in for a Closer Look
Technical advances allow analysis of signaling events at the level of single cells and individual molecules.
Perspectives
- The Impact of X-ray Crystallography and NMR on Intracellular Calcium Signal Transduction by EF-Hand Proteins: Crossing the Threshold from Structure to Biology and Medicine
Analysis of EF-hand proteins in complexes with their targets may define the structural basis for functional consequences.
- Parsing the Motion of Single Molecules: A Novel Algorithm for Deconvoluting the Dynamics of Individual Receptors at the Cell Surface
A new statistical method helps distinguish a protein moving with a purpose from a protein on a random walk.
- Microfluidic Devices for the Analysis of Single Cells: Leaving No Protein Uncounted
A microfluidic device counts the number of low abundance proteins in a single cell.
Editors' Choice
- The Arylhydrocarbon Receptor Sees the Light
The arylhydrocarbon receptor (AhR) mediates the cytosolic response of cells to ultraviolet (UVB) irradiation.
- Setting the Metabolic Beat
The transcriptional coactivator PGC-1α plays a central role in coordinating the circadian cycling with energy metabolism.
- NEMO: Master of Interferon Production
NEMO allows infected cells to activate the transcription factors NF-κB and interferon regulatory factors (IRFs) to mount an efficient antiviral response.
- TLR2 Stimulates Th1 Effector Functions
Ligands for Toll-like receptor 2 (TLR2), but not other TLRs, can stimulate the production of interferon-γ by Th1 effector cells.
- Nuclear Shut Off of NF-κB
PDLIM2 is a nuclear E3 ubiquitin ligase that targets a subunit of NF-κB to PML bodies for degradation.
- Shifting into Reverse
Inhibition of phospholipase C activity reverses the polarity of phosphatidylinositol-(4,5) phosphate, thereby allowing cells to move away from a chemorepellent.
- R-spondins Signal Like Wnts, Sort Of
R-spondins regulate development through a distinct mode of activation of Wnt receptors
- DNA Damage-Response Teams
A proteomic analysis reveals that DNA damage induces phosphorylation of hundreds of proteins, many of which were not previously associated with this response.
- Real-Life Transcription Factor Dynamics
A repressor protein in Escherichia coli spends about 90% of its time diffusing along DNA, falling off, and trying again if it does not find its specific binding site within 5 milliseconds.
- Marijuana and Developmental Damage
Endocannabinoids can inhibit the growth of axons; mice without one type of cannabinoid receptor have more inhibitory neuronal inputs to cortical areas of the brain.
