System-Wide Temporal Characterization of the Proteome and Phosphoproteome of Human Embryonic Stem Cell Differentiation

Kristoffer T. G. Rigbolt; Tatyana A. Prokhorova; Vyacheslav Akimov; Jeanette Henningsen; Pia T. Johansen; Irina Kratchmarova; Moustapha Kassem; Matthias Mann; Jesper V. Olsen; Blagoy Blagoev

doi:10.1126/scisignal.2001570

Research ResourcePhosphoproteomics

System-Wide Temporal Characterization of the Proteome and Phosphoproteome of Human Embryonic Stem Cell Differentiation

Kristoffer T. G. Rigbolt¹,*,
Tatyana A. Prokhorova¹,*,
Vyacheslav Akimov¹,
Jeanette Henningsen¹,²,
Pia T. Johansen³,
Irina Kratchmarova¹,
Moustapha Kassem³,⁴,
Matthias Mann⁵,⁶,
Jesper V. Olsen⁶,†, and
Blagoy Blagoev¹,†

¹Center for Experimental BioInformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.
²Centre of Inflammation and Metabolism, University of Copenhagen, Rigshospitalet, Blegdamsvej 9, DK-2100 Copenhagen, Denmark.
³Molecular Endocrinology Laboratory, Department of Endocrinology, Odense University Hospital and Medical Biotechnology Center, Winslowsparken 25, DK-5000 Odense, Denmark.
⁴Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, 11461 Riyadh, Kingdom of Saudi Arabia.
⁵Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
⁶Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3b, DK-2200 Copenhagen, Denmark.

↵†To whom correspondence should be addressed. E-mail: bab{at}bmb.sdu.dk (B.B.); jesper.olsen{at}cpr.ku.dk (J.V.O.)

↵* These authors contributed equally to this work.

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Science Signaling 15 Mar 2011:
Vol. 4, Issue 164, pp. rs3
DOI: 10.1126/scisignal.2001570

Kristoffer T. G. Rigbolt

1Center for Experimental BioInformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.

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Tatyana A. Prokhorova

1Center for Experimental BioInformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.

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Vyacheslav Akimov

1Center for Experimental BioInformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.

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Jeanette Henningsen

1Center for Experimental BioInformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.

2Centre of Inflammation and Metabolism, University of Copenhagen, Rigshospitalet, Blegdamsvej 9, DK-2100 Copenhagen, Denmark.

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Pia T. Johansen

3Molecular Endocrinology Laboratory, Department of Endocrinology, Odense University Hospital and Medical Biotechnology Center, Winslowsparken 25, DK-5000 Odense, Denmark.

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Irina Kratchmarova

1Center for Experimental BioInformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.

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Moustapha Kassem

3Molecular Endocrinology Laboratory, Department of Endocrinology, Odense University Hospital and Medical Biotechnology Center, Winslowsparken 25, DK-5000 Odense, Denmark.

4Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, 11461 Riyadh, Kingdom of Saudi Arabia.

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Matthias Mann

5Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.

6Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3b, DK-2200 Copenhagen, Denmark.

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Jesper V. Olsen

6Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3b, DK-2200 Copenhagen, Denmark.

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For correspondence: bab@bmb.sdu.dk jesper.olsen@cpr.ku.dk

Blagoy Blagoev

1Center for Experimental BioInformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.

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For correspondence: bab@bmb.sdu.dk jesper.olsen@cpr.ku.dk

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Dynamic phosphorylation during stem cell differentiation may control recruitment of DNA methyltransferases to silence genes that maintain pluripotency.

System-Wide Temporal Characterization of the Proteome and Phosphoproteome of Human Embryonic Stem Cell Differentiation

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