Editors' ChoiceCell Biology

Mammalian DNA Damage Response

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Science Signaling  14 Jun 2011:
Vol. 4, Issue 177, pp. ec168
DOI: 10.1126/scisignal.4177ec168

To identify previously unrecognized components of a mammalian DNA damage response system, Cotta-Ramusino et al. used a large-scale screen of small interfering RNAs in a human tumor cell line. A group of about 100 genes were identified that were necessary to keep cells with damaged DNA from proceeding into mitosis. Two of the proteins that localized at sites of DNA damage were CLOCK and RHINO. CLOCK is a transcription factor that functions as part of the circadian clock, and RHINO is a component of a complex of proteins that act together to stimulate activity of the protein kinase ATR, which propagates the signal to arrest the cell cycle and allow DNA repair.

C. Cotta-Ramusino, E. R. McDonald III, K. Hurov, M. E. Sowa, J. W. Harper, S. J. Elledge, A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling. Science 332, 1313–1317 (2011). [Abstract] [Full Text]

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