Research ResourcePhosphoproteomics

Quantitative Phosphoproteomics Identifies Substrates and Functional Modules of Aurora and Polo-Like Kinase Activities in Mitotic Cells

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Science Signaling  28 Jun 2011:
Vol. 4, Issue 179, pp. rs5
DOI: 10.1126/scisignal.2001497

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Building the Corpus of Substrates of Mitotic Kinases

Mitosis is a complex process involving duplication of DNA, nuclear membrane dissolution, construction of a mitotic spindle, proper segregation of chromosomes, and, finally, creation of two new cells—each with a complete set of genomic material. Protein phosphorylation plays a critical role in this process and is mediated predominantly by three sets of kinases: the cyclin-dependent kinase–cyclin complex Cdk1/cyclinB, the Aurora family (Aurora A and B), and the Polo-like kinase (Plk) family, especially Plk1. To explore the targets of these kinases, Kettenbach et al. combined specific small-molecule kinase inhibitors with large-scale quantitative phosphoproteomics of mitotic mammalian cells. Their data enable refinement of the motifs recognized by these kinases and suggest previously unknown functions for these kinases, as well as serve as a useful resource for future exploration of these essential mitotic regulators.