Sequence-Specific Recognition of a PxLPxI/L Motif by an Ankyrin Repeat Tumbler Lock

Chao Xu; Jing Jin; Chuanbing Bian; Robert Lam; Ruijun Tian; Ryan Weist; Linya You; Jianyun Nie; Alexey Bochkarev; Wolfram Tempel; Chris S. Tan; Gregory A. Wasney; Masoud Vedadi; Gerald D. Gish; Cheryl H. Arrowsmith; Tony Pawson; Xiang-Jiao Yang; Jinrong Min

doi:10.1126/scisignal.2002979

Research ArticleStructural Biology

Sequence-Specific Recognition of a PxLPxI/L Motif by an Ankyrin Repeat Tumbler Lock

Chao Xu¹,*,
Jing Jin²,*,
Chuanbing Bian¹,
Robert Lam¹,†,
Ruijun Tian²,
Ryan Weist³,⁴,
Linya You³,⁴,
Jianyun Nie³,⁵,
Alexey Bochkarev¹,
Wolfram Tempel¹,
Chris S. Tan²,
Gregory A. Wasney¹,
Masoud Vedadi¹,
Gerald D. Gish²,
Cheryl H. Arrowsmith¹,⁶,
Tony Pawson²,⁷,
Xiang-Jiao Yang³,⁴, and
Jinrong Min¹,⁸,‡

¹Structural Genomics Consortium, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
²Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada.
³Rosalind and Morris Goodman Cancer Research Center and Department of Biochemistry, McGill University, Montréal, Québec H3A 1A3, Canada.
⁴Department of Medicine, McGill University Health Center, Montréal, Québec H3A 1A3, Canada.
⁵The Third Affiliated Hospital, Kunming Medical College, Yunnan 650031, China.
⁶Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 1L7, Canada.
⁷Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
⁸Department of Physiology, University of Toronto, Toronto, Ontario M5S 1A8, Canada.

↵‡To whom correspondence should be addressed. E-mail: jr.min{at}utoronto.ca

↵* These authors contributed equally to this work.
↵† Present address: Ontario Cancer Institute, Princess Margaret Hospital, University Health Network, 610 University Avenue, Toronto, Ontario M5G 2M9, Canada.

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Science Signaling 29 May 2012:
Vol. 5, Issue 226, pp. ra39
DOI: 10.1126/scisignal.2002979

Chao Xu

1Structural Genomics Consortium, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.

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Jing Jin

2Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada.

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Chuanbing Bian

1Structural Genomics Consortium, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.

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Robert Lam

1Structural Genomics Consortium, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.

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Ruijun Tian

2Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada.

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Ryan Weist

3Rosalind and Morris Goodman Cancer Research Center and Department of Biochemistry, McGill University, Montréal, Québec H3A 1A3, Canada.

4Department of Medicine, McGill University Health Center, Montréal, Québec H3A 1A3, Canada.

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Linya You

3Rosalind and Morris Goodman Cancer Research Center and Department of Biochemistry, McGill University, Montréal, Québec H3A 1A3, Canada.

4Department of Medicine, McGill University Health Center, Montréal, Québec H3A 1A3, Canada.

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Jianyun Nie

3Rosalind and Morris Goodman Cancer Research Center and Department of Biochemistry, McGill University, Montréal, Québec H3A 1A3, Canada.

5The Third Affiliated Hospital, Kunming Medical College, Yunnan 650031, China.

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Alexey Bochkarev

1Structural Genomics Consortium, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.

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Wolfram Tempel

1Structural Genomics Consortium, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.

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Chris S. Tan

2Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada.

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Gregory A. Wasney

1Structural Genomics Consortium, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.

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Masoud Vedadi

1Structural Genomics Consortium, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.

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Gerald D. Gish

2Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada.

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Cheryl H. Arrowsmith

1Structural Genomics Consortium, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.

6Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 1L7, Canada.

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Tony Pawson

2Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada.

7Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.

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Xiang-Jiao Yang

3Rosalind and Morris Goodman Cancer Research Center and Department of Biochemistry, McGill University, Montréal, Québec H3A 1A3, Canada.

4Department of Medicine, McGill University Health Center, Montréal, Québec H3A 1A3, Canada.

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Jinrong Min

1Structural Genomics Consortium, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.

8Department of Physiology, University of Toronto, Toronto, Ontario M5S 1A8, Canada.

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For correspondence: jr.min@utoronto.ca

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