Research ArticleImmunology

The Chemokine CXCL12 Generates Costimulatory Signals in T Cells to Enhance Phosphorylation and Clustering of the Adaptor Protein SLP-76

See allHide authors and affiliations

Science Signaling  30 Jul 2013:
Vol. 6, Issue 286, pp. ra65
DOI: 10.1126/scisignal.2004018

You are currently viewing the editor's summary.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution

Chemokine Enhancement of TCR Signals

Stimulation of the T cell receptor (TCR) activates multiple receptor-proximal signals, including the phosphorylation and clustering of the adaptor protein SLP-76. In addition to stimulating the chemotaxis of T cells through its G protein–coupled receptor CXCR4, the chemokine CXCL12 acts as a costimulatory signal for T cells. Smith et al. performed live confocal imaging of TCR-stimulated human T cells and found that simultaneous stimulation with CXCL12 enhanced the number, stability, and phosphorylation of SLP-76 microclusters and the expression of TCR target genes. Mutation of either of two critical tyrosine residues in SLP-76 blocked this effect of CXCL12, as did inhibition of the coupling of CXCR4 to Gi-family G proteins. These data suggest that T cells simultaneously exposed to CXCL12 and antigen are enhanced in activation, thereby boosting the immune response.

View Full Text

Stay Connected to Science Signaling