Research ResourceMOLECULAR BIOLOGY

Simultaneous Profiling of 194 Distinct Receptor Transcripts in Human Cells

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Science Signaling  06 Aug 2013:
Vol. 6, Issue 287, pp. rs13
DOI: 10.1126/scisignal.2003624

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Taking Receptor Roll Call

The quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay represents the gold standard for analyzing transcript abundance accurately. Kang et al. developed a set of primers and methods for performing parallel qRT-PCR analysis of 194 receptor-encoding genes, which represent a large proportion of cell surface receptors outside of the G protein (heterotrimeric guanine nucleotide–binding protein)–coupled receptor superfamily. Using this “receptome profiling” approach, the authors analyzed 40 human cells from breast, colon, pancreas, and melanoma and identified lineage-specific receptor profiles. Receptor absence was an indicator of lineage specificity and also functioned to prevent inappropriate autocrine signaling. This sensitive, specific, and inexpensive method should be a valuable tool in distinguishing which ligands will stimulate a cell and which will be ignored.

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