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mMAPS: A Flow-Proteometric Technique to Analyze Protein-Protein Interactions in Individual Signaling Complexes

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Science Signaling  04 Mar 2014:
Vol. 7, Issue 315, pp. rs1
DOI: 10.1126/scisignal.2004620

Quantifying Individual Signaling Complexes

Biomolecular interactions, such as protein-protein and protein-DNA interactions, transmit regulatory information in cells. To quantitatively analyze signaling interactions that occur in individual protein-containing complexes, Chou et al. developed a technique called microchannel for multiparameter analysis of proteins in a single complex (mMAPS). With this method, which relies on labeling proteins of interest with fluorochromes and separating the labeled complexes by flow proteometry, molecular interactions within a single protein-protein complex in lysates from cultured cells or tumor tissue were quantified. Application of this technique may produce quantitative insight into the dynamics of molecular interactions.

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