Research ArticleSystems Biology

Phosphoproteomic analysis identifies proteins involved in transcription-coupled mRNA decay as targets of Snf1 signaling

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Science Signaling  08 Jul 2014:
Vol. 7, Issue 333, pp. ra64
DOI: 10.1126/scisignal.2005000

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Cells Adapt with Snf1 and Xrn1

Cells adapt to starvation in part through activation of the AMPK family of protein kinases. In yeast, the AMPK family member Snf1 responds to the lack of glucose by phosphorylating various proteins, including those that activate transcription of genes involved in the consumption of other nutrient sources. Braun et al. analyzed the proteome for changes in phosphorylation in normal and Snf1 activity–deficient yeast grown under glucose-deficient conditions and found Snf1-dependent changes in more than 100 proteins, including several proteins involved in the synthesis and decay of mRNA. One such protein known as Xrn1, which may couple mRNA synthesis and decay, was required for the activation of Snf1-dependent genes in the absence of glucose and their subsequent degradation when yeast were fed with glucose. Understanding Snf1-regulated proteins in yeast could inform studies in human cellular nutrient metabolism.

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