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An analysis of critical factors for quantitative immunoblotting

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Science Signaling  07 Apr 2015:
Vol. 8, Issue 371, pp. rs2
DOI: 10.1126/scisignal.2005966

Best practices for immunoblotting

Immunoblotting, also known as Western blotting, is a common technique used by cell and molecular biologists to detect the presence of proteins, changes in protein abundance, and the presence and changes in abundance of posttranslational modifications. Unfortunately, many factors influence reproducibility and the ability to produce quantitative results with this technique. Janes provides a detailed analysis of how variations in sample preparation, detection scheme, and normalization approach affect the reliability, reproducibility, and quantitative value of proteins evaluated by immunoblotting.