Research ResourceBiochemistry

Rapid multiplex analysis of lipid raft components with single-cell resolution

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Science Signaling  22 Sep 2015:
Vol. 8, Issue 395, pp. rs11
DOI: 10.1126/scisignal.aac5584

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Lipid raft analysis by flow cytometry

Lipid rafts are dynamic regions of membranes that are involved in cell signaling but are challenging to study because of their small size and dynamic nature. Schatzlmaier et al. found that lipid raft components became associated with nuclei during lysis of cells as the cells passed through a detergent-containing layer in a gradient. In experiments with lymphocytes, the authors demonstrated that this association enabled the quantitative analysis by flow cytometry of the composition of lipid rafts and of the dynamic association of proteins with these membrane microdomains at single-cell resolution.