Research ArticleCell Migration

Dynamic regulation of neutrophil polarity and migration by the heterotrimeric G protein subunits Gαi-GTP and Gβγ

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Science Signaling  23 Feb 2016:
Vol. 9, Issue 416, pp. ra22
DOI: 10.1126/scisignal.aad8163

Control cAMP to control migration

Activation of the G protein–coupled receptors (GPCRs) that stimulate cellular migration generates active G protein α and βγ subunits, which interact with distinct effector molecules. Using a small molecule that activates βγ subunits without activating α subunits in neutrophils, Surve et al. determined that active βγ subunits alone increased the intracellular concentration of the second messenger cAMP so much that the cells stuck to coated surfaces. Active G protein αi subunits balanced this βγ signal, reducing cAMP sufficiently to enable the cells to move.

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