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PLIF: A rapid, accurate method to detect and quantitatively assess protein-lipid interactions

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Science Signaling  29 Mar 2016:
Vol. 9, Issue 421, pp. rs2
DOI: 10.1126/scisignal.aad4337

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Detecting lipid-protein partners with PLIF

Phosphoinositides are a group of low-abundance lipid molecules in cellular membranes that regulate cellular physiology. Aberrant signaling in the pathways mediated by phosphoinositides underlies a wide range of diseases, including cancer, obesity, and neurodegenerative disorders. Ceccato et al. developed a method that enables fast and sensitive detection of phosphoinositide-binding partners than currently available approaches. The fluorescence-based method PLIF not only detected known phosphoinositide partners for various proteins but also determined relative binding affinities. The authors used their method to show that sorting nexins, which mediate vesicular trafficking, are promiscuous, interacting with many types of phosphoinositides. The high-throughput nature of PLIF makes it directly applicable to drug development aimed at disrupting protein-lipid interactions.

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