Research ResourcePlant biology Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission–Brillouin imaging Kareem Elsayad1,*,†, Stephanie Werner2,*,‡, Marçal Gallemí2,§, Jixiang Kong2, Edmundo R. Sánchez Guajardo1, Lijuan Zhang1, Yvon Jaillais3, Thomas Greb2,¶, and Youssef Belkhadir2,*,†1Advanced Microscopy Facility, Vienna Biocenter Core Facilities, A-1030 Vienna, Austria.2Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, A-1030 Vienna, Austria.3Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342 Lyon, France.↵†Corresponding author. Email: kareem.elsayad{at}vbcf.ac.at (K.E.); youssef.belkhadir{at}gmi.oeaw.ac.at (Y.B.)↵* These authors contributed equally to this work (joint first authors).↵* Present address: Department of Forest Botany and Tree Physiology, University of Göttingen, 37077 Göttingen, Germany.↵§ Present address: Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.↵¶ Present address: Center for Organismal Studies, Heidelberg University, 69120 Heidelberg, Germany. See allHide authors and affiliations Science Signaling 05 Jul 2016:Vol. 9, Issue 435, pp. rs5DOI: 10.1126/scisignal.aaf6326 Kareem Elsayad 1Advanced Microscopy Facility, Vienna Biocenter Core Facilities, A-1030 Vienna, Austria.Find this author on Google Scholar Find this author on PubMed Search for this author on this site For correspondence: kareem.elsayad@vbcf.ac.at youssef.belkhadir@gmi.oeaw.ac.at Stephanie Werner 2Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, A-1030 Vienna, Austria.Find this author on Google Scholar Find this author on PubMed Search for this author on this site Marçal Gallemí 2Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, A-1030 Vienna, Austria.Find this author on Google Scholar Find this author on PubMed Search for this author on this site Jixiang Kong 2Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, A-1030 Vienna, Austria.Find this author on Google Scholar Find this author on PubMed Search for this author on this site Edmundo R. Sánchez Guajardo 1Advanced Microscopy Facility, Vienna Biocenter Core Facilities, A-1030 Vienna, Austria.Find this author on Google Scholar Find this author on PubMed Search for this author on this site Lijuan Zhang 1Advanced Microscopy Facility, Vienna Biocenter Core Facilities, A-1030 Vienna, Austria.Find this author on Google Scholar Find this author on PubMed Search for this author on this site Yvon Jaillais 3Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342 Lyon, France.Find this author on Google Scholar Find this author on PubMed Search for this author on this site Thomas Greb 2Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, A-1030 Vienna, Austria.Find this author on Google Scholar Find this author on PubMed Search for this author on this site Youssef Belkhadir 2Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, A-1030 Vienna, Austria.Find this author on Google Scholar Find this author on PubMed Search for this author on this site For correspondence: kareem.elsayad@vbcf.ac.at youssef.belkhadir@gmi.oeaw.ac.at Article Figures & Data Info & Metrics eLetters PDF Article Information vol. 9 no. 435 rs5 DOI: https://doi.org/10.1126/scisignal.aaf6326 PubMed: 27382028 Published By: Science Signaling Print ISSN: 1945-0877 Online ISSN: 1937-9145 History: Received March 8, 2016Accepted June 17, 2016 . Copyright & Usage: Copyright © 2016, American Association for the Advancement of Science Author Information Altmetric Article usage Article lifetimeLast 6 monthsThis monthArticle usage: June 2016 to February 2021 AbstractFullPdf Jun 2016611 Jul 20166543336456 Aug 20166434265 Sep 20163462853 Oct 20163452032 Nov 20162944250 Dec 20161391634 Jan 20171502723 Feb 20171061217 Mar 20171142232 Apr 2017931531 May 2017831628 Jun 2017672517 Jul 2017701413 Aug 2017861819 Sep 2017831934 Oct 20171241716 Nov 2017922014 Dec 201761107 Jan 2018911018 Feb 20181191416 Mar 2018951210 Apr 20181151615 May 2018961422 Jun 20184684 Jul 2018631013 Aug 201855284 Sep 20181053216 Oct 201896209 Nov 20181523517 Dec 20181044118 Jan 2019943815 Feb 2019771712 Mar 20191023316 Apr 2019701911 May 2019682216 Jun 201942156 Jul 2019662616 Aug 201954224 Sep 2019883310 Oct 2019723822 Nov 2019621610 Dec 201944145 Jan 2020563611 Feb 202045248 Mar 202041154 Apr 2020721210 May 2020521911 Jun 202043157 Jul 202036134 Aug 202035248 Sep 2020583014 Oct 202059239 Nov 2020722716 Dec 2020593920 Jan 2021602710 Feb 2021683211 View Full Text
Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission–Brillouin imaging By Kareem Elsayad, Stephanie Werner, Marçal Gallemí, Jixiang Kong, Edmundo R. Sánchez Guajardo, Lijuan Zhang, Yvon Jaillais, Thomas Greb, Youssef Belkhadir Science Signaling05 Jul 2016 : rs5 Fluorescence-Brillouin imaging reveals that plants regulate the mechanical properties of the extracellular matrix in response to light. Supplementary Materials
Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission–Brillouin imaging By Kareem Elsayad, Stephanie Werner, Marçal Gallemí, Jixiang Kong, Edmundo R. Sánchez Guajardo, Lijuan Zhang, Yvon Jaillais, Thomas Greb, Youssef Belkhadir Science Signaling05 Jul 2016 : rs5 Fluorescence-Brillouin imaging reveals that plants regulate the mechanical properties of the extracellular matrix in response to light. Supplementary Materials