Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission–Brillouin imaging

Kareem Elsayad; Stephanie Werner; Marçal Gallemí; Jixiang Kong; Edmundo R. Sánchez Guajardo; Lijuan Zhang; Yvon Jaillais; Thomas Greb; Youssef Belkhadir

doi:10.1126/scisignal.aaf6326

Research ResourcePlant biology

Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission–Brillouin imaging

Kareem Elsayad¹,*,†,
Stephanie Werner²,*,‡,
Marçal Gallemí²,§,
Jixiang Kong²,
Edmundo R. Sánchez Guajardo¹,
Lijuan Zhang¹,
Yvon Jaillais³,
Thomas Greb²,¶, and
Youssef Belkhadir²,*,†

¹Advanced Microscopy Facility, Vienna Biocenter Core Facilities, A-1030 Vienna, Austria.
²Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, A-1030 Vienna, Austria.
³Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342 Lyon, France.

↵†Corresponding author. Email: kareem.elsayad{at}vbcf.ac.at (K.E.); youssef.belkhadir{at}gmi.oeaw.ac.at (Y.B.)

↵* These authors contributed equally to this work (joint first authors).
↵* Present address: Department of Forest Botany and Tree Physiology, University of Göttingen, 37077 Göttingen, Germany.
↵§ Present address: Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
↵¶ Present address: Center for Organismal Studies, Heidelberg University, 69120 Heidelberg, Germany.

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Science Signaling 05 Jul 2016:
Vol. 9, Issue 435, pp. rs5
DOI: 10.1126/scisignal.aaf6326

Kareem Elsayad

1Advanced Microscopy Facility, Vienna Biocenter Core Facilities, A-1030 Vienna, Austria.

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For correspondence: kareem.elsayad@vbcf.ac.at youssef.belkhadir@gmi.oeaw.ac.at

Stephanie Werner

2Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, A-1030 Vienna, Austria.

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Marçal Gallemí

2Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, A-1030 Vienna, Austria.

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Jixiang Kong

2Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, A-1030 Vienna, Austria.

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Edmundo R. Sánchez Guajardo

1Advanced Microscopy Facility, Vienna Biocenter Core Facilities, A-1030 Vienna, Austria.

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Lijuan Zhang

1Advanced Microscopy Facility, Vienna Biocenter Core Facilities, A-1030 Vienna, Austria.

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Yvon Jaillais

3Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342 Lyon, France.

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Thomas Greb

2Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, A-1030 Vienna, Austria.

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Youssef Belkhadir

2Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, A-1030 Vienna, Austria.

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For correspondence: kareem.elsayad@vbcf.ac.at youssef.belkhadir@gmi.oeaw.ac.at

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Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission–Brillouin imaging

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