Bimolecular complementation affinity purification (BiCAP) reveals dimer-specific protein interactions for ERBB2 dimers

David R. Croucher; Mary Iconomou; Jordan F. Hastings; Sean P. Kennedy; Jeremy Z. R. Han; Robert F. Shearer; Jessie McKenna; Adrian Wan; Joseph Lau; Samuel Aparicio; Darren N. Saunders

doi:10.1126/scisignal.aaf0793

Research ArticleCell Biology

Bimolecular complementation affinity purification (BiCAP) reveals dimer-specific protein interactions for ERBB2 dimers

David R. Croucher¹,²,³,*,
Mary Iconomou¹,†,
Jordan F. Hastings¹,†,
Sean P. Kennedy¹,⁴,†,
Jeremy Z. R. Han¹,
Robert F. Shearer¹,
Jessie McKenna¹,
Adrian Wan⁵,
Joseph Lau⁵,
Samuel Aparicio⁵,⁶, and
Darren N. Saunders¹,⁷,*

¹The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia.
²St. Vincent’s Hospital Clinical School, University of New South Wales, Sydney, New South Wales 2052, Australia.
³School of Medicine, University College Dublin, Belfield, Dublin D4, Ireland.
⁴Systems Biology Ireland, University College Dublin, Belfield, Dublin D4, Ireland.
⁵Department of Molecular Oncology, British Columbia Cancer Research Centre, Vancouver, British Columbia V5Z 1L3, Canada.
⁶Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
⁷School of Medical Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia.

↵*Corresponding author. Email: d.croucher{at}garvan.org.au (D.R.C.); d.saunders{at}unsw.edu.au (D.N.S.)

↵† These authors contributed equally to this work.

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Science Signaling 12 Jul 2016:
Vol. 9, Issue 436, pp. ra69
DOI: 10.1126/scisignal.aaf0793

David R. Croucher

1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia.

2St. Vincent’s Hospital Clinical School, University of New South Wales, Sydney, New South Wales 2052, Australia.

3School of Medicine, University College Dublin, Belfield, Dublin D4, Ireland.

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For correspondence: d.croucher@garvan.org.au d.saunders@unsw.edu.au

Mary Iconomou

1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia.

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Jordan F. Hastings

1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia.

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Sean P. Kennedy

1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia.

4Systems Biology Ireland, University College Dublin, Belfield, Dublin D4, Ireland.

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Jeremy Z. R. Han

1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia.

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Robert F. Shearer

1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia.

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Jessie McKenna

1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia.

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Adrian Wan

5Department of Molecular Oncology, British Columbia Cancer Research Centre, Vancouver, British Columbia V5Z 1L3, Canada.

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Joseph Lau

5Department of Molecular Oncology, British Columbia Cancer Research Centre, Vancouver, British Columbia V5Z 1L3, Canada.

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Samuel Aparicio

5Department of Molecular Oncology, British Columbia Cancer Research Centre, Vancouver, British Columbia V5Z 1L3, Canada.

6Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.

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Darren N. Saunders

1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia.

7School of Medical Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia.

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For correspondence: d.croucher@garvan.org.au d.saunders@unsw.edu.au

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Bimolecular complementation affinity purification (BiCAP) reveals dimer-specific protein interactions for ERBB2 dimers

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