Supplementary Materials for:

AAA+ Proteins RUVBL1 and RUVBL2 Coordinate PIKK Activity and Function in Nonsense-Mediated mRNA Decay

Natsuko Izumi, Akio Yamashita,* Akihiro Iwamatsu, Rie Kurata, Hiroki Nakamura, Bonnie Saari, Hisashi Hirano, Philip Anderson, Shigeo Ohno*

*To whom correspondence should be addressed. E-mail: ohnos{at}med.yokohama-cu.ac.jp (S.O.); yamasita{at}yokohama-cu.ac.jp (A.Y.)

This PDF file includes:

• Fig. S1. RUVBL1, RUVBL2, SMG-10, and RPB5 form a complex.
• Fig. S2. Time course analysis of the effect of the RUVBL1 or RUVBL2 knockdown on PIKK abundance.
• Fig. S3. Treatment with proteolysis inhibitors did not restore the decreased PIKK abundance caused by the knockdown of RUVBL1 or RUVBL2.
• Fig. S4. Knockdown of SMG-10 or RPB5 does not have notable effects on the PIKK abundance.
• Fig. S5. RUVBL1 is required for down-regulation of the natural targets of nonsense-mediated mRNA decay.
• Fig. S6. RPB5 is involved in nonsense-mediated mRNA decay in mammalian cells.
• Table S1. RUVBL1 interacting proteins identified by LC-MS/MS analysis of affinity-purified, SBP-tagged RUVBL1 complexes.

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Citation: N. Izumi, A. Yamashita, A. Iwamatsu, R. Kurata, H. Nakamura, B. Saari, H. Hirano, P. Anderson, S. Ohno, AAA+ Proteins RUVBL1 and RUVBL2 Coordinate PIKK Activity and Function in Nonsense-Mediated mRNA Decay. Sci. Signal. 3, ra27 (2010).

© 2010 American Association for the Advancement of Science