Supplementary Materials

Supplementary Materials for:

Eukaryotic G Protein Signaling Evolved to Require G Protein–Coupled Receptors for Activation

William Bradford, Adam Buckholz, John Morton, Collin Price, Alan M. Jones,* Daisuke Urano

*Corresponding author. E-mail: alan_jones{at}unc.edu

This PDF file includes:

  • Fig. S1. Genes encoding heterotrimeric G protein components found in eukaryotic genomes.
  • Fig. S2. Phylogeny of unikonta GPCRs.
  • Fig. S3. Minimum spanning network for unikonta GPCRs.
  • Fig. S4. ML trees of the 7TM-RGS proteins show the distinct origin of 7TM-RGS proteins.
  • Fig. S5. Intrinsic fluorescence measurements of the nucleotide exchange and hydrolysis activities of Gα proteins support the radionucleotide assay–based measurements.
  • Fig. S6. Identity among T. vaginalis G protein components.
  • Fig. S7. Multiple sequence alignment of Gα subunits showing conserved residues.
  • Table S1. Accession numbers of genes encoding G proteins, RGS proteins, and GPCRs.
  • References (43–47)

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Citation: W. Bradford, A. Buckholz, J. Morton, C. Price, A. M. Jones, D. Urano, Eukaryotic G Protein Signaling Evolved to Require G Protein–Coupled Receptors for Activation. Sci. Signal. 6, ra37 (2013).

© 2013 American Association for the Advancement of Science