Supplementary Materials

Supplementary Materials for:

TGF-β Induces Acetylation of Chromatin and of Ets-1 to Alleviate Repression of miR-192 in Diabetic Nephropathy

Mitsuo Kato,* Varun Dang, Mei Wang, Jung Tak Park, Supriya Deshpande, Swati Kadam, Armen Mardiros, Yumei Zhan, Peter Oettgen, Sumanth Putta, Hang Yuan, Linda Lanting, Rama Natarajan*

*Corresponding author. E-mail: mkato@coh.org (M.K.); rnatarajan{at}coh.org (R.N.)

This PDF file includes:

  • Fig. S1. Effects of TGF-β antibody on the expression of miR-192, other miRNAs, and fibrotic factors in MCs treated with high glucose.
  • Fig. S2. Western blots of Ets-1 and pEts-1 in MCs treated with TGF-β.
  • Fig. S3. Sustained acetylation of Ets-1 up to 72 hours after TGF-β treatment and its inhibition by MK-2206.
  • Fig. S4. Western blots of p-Akt (Ser473) in MCs treated with TGF-β and transfected with dominant-negative Akt.
  • Fig. S5. Effects of MK-2206 on the expression of miR-192 host RNA CJ24 and collagens in murine MCs treated with TGF-β.
  • Fig. S6. Reporter activity in response to TGF-β in MCs derived from Ets-1–deficient mice.
  • Fig. S7. Decreased enrichment of acetyl Ets-1 compared to Ets-1 in the upstream region of miR-192.
  • Fig. S8. Effects of miR-192 inhibitor on Ets-1 occupancy at Smad or Ets-1 sites in the upstream region of miR-192 in MCs.

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Citation: M. Kato, V. Dang, M. Wang, J. T. Park, S. Deshpande, S. Kadam, A. Mardiros, Y. Zhan, P. Oettgen, S. Putta, H. Yuan, L. Lanting, R. Natarajan, TGF-β Induces Acetylation of Chromatin and of Ets-1 to Alleviate Repression of miR-192 in Diabetic Nephropathy. Sci. Signal. 6, ra43 (2013).

© 2013 American Association for the Advancement of Science