Supplementary Materials

Supplementary Materials for:

In Vivo Phosphoproteomics Analysis Reveals the Cardiac Targets of β-Adrenergic Receptor Signaling

Alicia Lundby,* Martin N. Andersen, Annette B. Steffensen, Heiko Horn, Christian D. Kelstrup, Chiara Francavilla, Lars J. Jensen, Nicole Schmitt, Morten B. Thomsen, Jesper V. Olsen*

*Corresponding author. E-mail: alicia.lundby@cpr.ku.dk (A.L.); jesper.olsen@cpr.ku.dk (J.V.O.)

This PDF file includes:

  • Fig. S1. Electrophysiological effects of pharmacological βAR modulation on murine hearts.
  • Fig. S2. Evaluation and assessment of MS and MS/MS data quality.
  • Fig. S3. Correlation plot analyses of biological replicates of phosphopeptide measurements.
  • Fig. S4. Statistical analysis of identified peptides.
  • Fig. S5. Western blotting analysis of Akt and GSK-3α in control and β1AR-stimulated mice.
  • Fig. S6. Localization of KV7.1 channels and presence of βARs in MDCK cells.
  • Fig. S7. Trafficking of KV7.1 Ser27 and Ser92 mutant channels in MDCK cells.
  • Fig. S8. Testing of KV7.1 wild-type, KV7.1 S92A, and KV7.1 S92D mutant channels coexpressed with KCNE1 in X. laevis oocytes.
  • Legends for tables S1 to S5

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). List of all identified modification-specific peptides.
  • Table S2 (Microsoft Excel format). List of all identified class 1 phosphorylation sites.
  • Table S3 (Microsoft Excel format). List of all identified proteins.
  • Table S4 (Microsoft Excel format). List of all phosphorylation sites regulated by β1AR stimulation.
  • Table S5 (Microsoft Excel format). List of all identified phosphorylated kinases.

Citation: A. Lundby,M. N. Andersen, A. B. Steffensen, H. Horn, C. D. Kelstrup, C. Francavilla, L. J. Jensen, N. Schmitt, M. B. Thomsen, J. V. Olsen, In Vivo Phosphoproteomics Analysis Reveals the Cardiac Targets of β-Adrenergic Receptor Signaling. Sci. Signal. 6, rs11 (2013).

© 2013 American Association for the Advancement of Science