Supplementary Materials

Supplementary Materials for:

Regulators of Calcium Homeostasis Identified by Inference of Kinetic Model Parameters from Live Single Cells Perturbed by siRNA

Samuel Bandara,* Seth Malmersjö, Tobias Meyer*

*Corresponding author. E-mail: sbandara@alumni.stanford.edu (S.B.); tobias1@stanford.edu (T.M.)

This PDF file includes:

  • Fig. S1. Characterization of parameter uncertainty.
  • Fig. S2. Increase in ER Ca2+ concentrations after knockdown of PSEN2 using different synthetic siRNA sequences.
  • Fig. S3. Effect of PSEN2 and ORAI2 overexpression on basal cytosolic Ca2+.
  • Fig. S4. Labeling of control cells with CMTPX to mark cells does not affect parameter estimates.
  • Legends for tables S1 and S2
  • Legend for Movie S1

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Technical Details

Format: Adobe Acrobat PDF

Size: 807 KB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Accession numbers and gene symbols of the focused siRNA library and primer sequences used for generating the diced pools from human cDNA.
  • Table S2 (Microsoft Excel format). Gene symbols of targeted proteins and corresponding log2-fold changes of the median single-cell parameter estimates compared to control cells assayed in the same well.
  • Movie S1 (.avi format). Movie of NCS1-mCherry in HeLa cells corresponding to Fig. 5A.

[Download Tables S1 to S2]

[Download Movie S1]


Citation: S. Bandara, S. Malmersjö, T. Meyer, Regulators of Calcium Homeostasis Identified by Inference of Kinetic Model Parameters from Live Single Cells Perturbed by siRNA. Sci. Signal. 6, ra56 (2013).

© 2013 American Association for the Advancement of Science