Supplementary Materials

Supplementary Materials for:

Transglutaminase-Catalyzed Protein-Protein Cross-Linking Suppresses the Activity of the NF-κB–Like Transcription Factor Relish

Toshio Shibata, Sanae Sekihara, Takumi Fujikawa, Ryuta Miyaji, Kouki Maki, Takeshi Ishihara, Takumi Koshiba, Shun-ichiro Kawabata*

*Corresponding author. E-mail: skawascb@kyudai.jp

This PDF file includes:

  • Fig. S1. Intracellular localization of TG.
  • Fig. S2. CFUs of gut lysates in sTG-RNAi flies.
  • Fig. S3. Survival rates after DCA ingestion.
  • Fig. S4. Diptericin expression in sTG-RNAi flies under conventional and germ-free conditions.
  • Fig. S5. Survival rates of Relish-overexpressing flies.
  • Fig. S6. Survival rates of conditional TG-knockdown flies.
  • Fig. S7. Intracellular localization of Relish in the gut of the control (Da>+) flies under germ-free conditions after Ecc15 or PBS ingestion.
  • Fig. S8. Analysis of the intracellular localization of Relish-N in S2 cells.
  • Fig. S9. Analysis of the intracellular localization of the BPA-incorporated protein in the middle midgut.
  • Fig. S10. TG-dependent incorporation of DCA into Relish-N in S2 cells.
  • Fig. S11. TG-RNAi induces peritrophic matrix defects.

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Citation: T. Shibata, S. Sekihara, T. Fujikawa, R. Miyaji, K. Maki, T. Ishihara, T. Koshiba, S.-i. Kawabata,Transglutaminase-Catalyzed Protein-Protein Cross-Linking Suppresses the Activity of the NF-κB–Like Transcription Factor Relish. Sci. Signal. 6, ra61 (2013).

© 2013 American Association for the Advancement of Science