Supplementary Materials

Supplementary Materials for:

Superresolution Microscopy Reveals Nanometer-Scale Reorganization of Inhibitory Natural Killer Cell Receptors upon Activation of NKG2D

Sophie V. Pageon, Shaun-Paul Cordoba, Dylan M. Owen, Stephen M. Rothery, Anna Oszmiana, Daniel M. Davis*

*Corresponding author. E-mail: daniel.davis@manchester.ac.uk

This PDF file includes:

  • Methods
  • Fig. S1. Functionality of KIR2DL1 and tdEosFP in NKL/KIR2DL1-tdEosFP cells.
  • Fig. S2. Functionality of KIR2DL1 and tdEosFP in YTS/KIR2DL1-tdEosFP cells.
  • Fig. S3. Ligation of NKG2D causes a spreading response in NKL/KIR2DL1-tdEosFP cells.
  • Fig. S4. GSDIM confirms the nanoscale reorganization of KIR2DL1 upon ligation of NKG2D.
  • Fig. S5. Comparison of cluster analysis by the circle and ring methods.
  • Fig. S6. Latrunculin A disrupts the actin cytoskeleton but does not inhibit activation in NKL/KIR2DL1-tdEosFP cells.
  • Fig. S7. Latrunculin A has no effect on the changes in organization of KIR2DL1 in YTS/KIR2DL1-tdEosFP cells induced by EB6.
  • Table S1. Quantification of the cell-surface abundance of KIR2DL1 molecules in NK cell line transfectants and primary human NK cells.
  • Table S2. Mean values for different parameters assessing KIR2DL1 distribution and cluster morphology.

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Citation: S. V. Pageon, S.-P. Cordoba, D. M. Owen, S. M. Rothery, A. Oszmiana, D. M. Davis, Superresolution Microscopy Reveals Nanometer-Scale Reorganization of Iinhibitory Natural Killer Cell Receptors upon Activation of NKG2D. Sci. Signal. 6, ra62 (2013).

© 2013 American Association for the Advancement of Science