Supplementary Materials

Supplementary Materials for:

miR-29 Acts as a Decoy in Sarcomas to Protect the Tumor Suppressor A20 mRNA from Degradation by HuR

Mumtaz Y. Balkhi,, O. Hans Iwenofu, Nadine Bakkar, Katherine J. Ladner, Dawn S. Chandler, Peter J. Houghton, Cheryl A. London, William Kraybill, Danilo Perrotti, Carlo M. Croce, Charles Keller, Denis C. Guttridge*

*Corresponding author. E-mail: denis.guttridge@osumc.edu

This PDF file includes:

  • Fig. S1. RIP1 activation and loss of A20 are associated with sarcoma cell lines.
  • Fig. S2. Abundance of A20 mRNA and protein is reduced in sarcomas.
  • Fig. S3. A20 is not frequently mutated in sarcomas.
  • Fig. S4. A20 regulation in sarcoma cells is not mediated at the transcriptional level.
  • Fig. S5. A20 is regulated by miR-29 and miR-125.
  • Fig. S6. Endogenous regulation of A20 and RIP1 by miR-29 promotes a differentiated phenotype in Rh30 cells.
  • Fig. S7. HuR inhibits the activity of the A20 3′UTR reporter.
  • Fig. S8. Putative HuR binding sites are present in the A20 3′UTR.
  • Fig. S9. Precursor miR-29 contains multiple HuR binding sites, and miR-125 association with Ago2 is dependent on HuR in sarcoma cells.
  • Table S1. Primers for the A20 3′UTR.
  • Table S2. Primers used to sequence A20 exons and UTRs.
  • Table S3. pRNAT-CMV3.1/hygro plasmids expressing wild-type or mutated miR-29 precursor sequences.
  • Table S4. Primers used for RT-PCR analysis.

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Citation: M. Y. Balkhi, O. H. Iwenofu, N. Bakkar, K. J. Ladner, D. S. Chandler, P. J. Houghton, C. A. London, W. Kraybill, D. Perrotti, C. M. Croce, C. Keller, D. C. Guttridge, miR-29 Acts as a Decoy in Sarcomas to Protect the Tumor Suppressor A20 mRNA from Degradation by HuR. Sci. Signal. 6, ra63 (2013).

© 2013 American Association for the Advancement of Science