Supplementary Materials
Supplementary Materials for:
Citation: B. H. Kang, K. J. Jensen, J. A. Hatch, K. A. Janes, Simultaneous Profiling of 194 Distinct Receptor Transcripts in Human Cells. Sci. Signal. 6, rs13 (2013).
Simultaneous Profiling of 194 Distinct Receptor Transcripts in Human Cells
Byong H. Kang, Karin J. Jensen, Jaime A. Hatch, Kevin A. Janes*
*Corresponding author. E-mail: kjanes@virginia.edu
This PDF file includes:
- Fig. S1. Receptome profiling is consistently reproducible across assay duplicates.
- Fig. S2. RNA-seq is consistently reproducible across assay duplicates with sensitivity that depends on sequencing depth.
- Fig. S3. Receptor discrepancies between qRT-PCR and RNA-seq in cancer cells are enriched for retained intronic sequences.
- Fig. S4. One-way hierarchical clustering of relative receptor abundances in 40 cell lines obtained by receptome profiling.
- Fig. S5. Two-way hierarchical clustering of relative receptor abundances in 40 cell lines obtained by receptome profiling.
- Fig. S6. One-way hierarchical clustering of high-sensitivity present-absent calls in 40 cell lines obtained by receptome profiling.
- Fig. S7. Two-way hierarchical clustering of high-sensitivity present-absent calls in 40 cell lines obtained by receptome profiling.
- Fig. S8. Changes in receptor abundance in 293T and MCF7 cells upon stimulation with EGF, IFN-γ, IR, and TNF.
- Fig. S9. Relative receptor abundance and present-absent calls in human brain and muscle tissue.
- Table S1. Literature support for present calls in HT-29 cells.
- Legends for files S1 to S6
- References (101–112)
Technical Details
Format: Adobe Acrobat PDF
Size: 1.78 MB
Other Supplementary Material for this manuscript includes the following:
- File S1 (Microsoft Excel format). Sequences of validated qRT-PCR primers for receptome profiling.
- File S2 (Microsoft Excel format). Comparison of present-absent calls obtained by receptome profiling and oligonucleotide microarrays for HT-29 cells exposed to IFN-γ.
- File S3 (Microsoft Excel format). Mean-centered cycle thresholds and present-absent calls of the signaling receptome in 40 human cell lines.
- File S4 (Microsoft Excel format). Response of 293T and MCF7 signaling receptomes to EGF, IFN-γ, IR, and TNF.
- File S5 (Microsoft Excel format). Signaling receptomes of primary human brain and muscle tissue.
- File S6 (.zip file). MATLAB software for present-absent calls and relative quantification.
Citation: B. H. Kang, K. J. Jensen, J. A. Hatch, K. A. Janes, Simultaneous Profiling of 194 Distinct Receptor Transcripts in Human Cells. Sci. Signal. 6, rs13 (2013).