Supplementary Materials

Supplementary Materials for:

A Computational Model Predicts That Gβγ Acts at a Cleft Between Channel Subunits to Activate GIRK1 Channels

Rahul Mahajan, Junghoon Ha, Miao Zhang, Takeharu Kawano, Tohru Kozasa, Diomedes E. Logothetis*

*Corresponding author. E-mail: delogothetis@vcu.edu

This PDF file includes:

  • Fig. S1. Permeation pathway, gates, and closed and open crystal forms of the intracellular G loop gate of a GIRK1 chimera.
  • Fig. S2. Comparison of our best-scoring computational model with an NMR model of the footprint of Gβγ on GIRK1 residues.
  • Fig. S3. Gβ- and GIRK1-interacting residues in the best-scoring and largest cluster models.
  • Fig. S4. Critical elements of the open versus closed G loop conformations of the channel structure.
  • Fig. S5. Largest cluster model details.
  • Fig. S6. Effect of channel mutations on mean currents relative to the G1*.

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Technical Details

Format: Adobe Acrobat PDF

Size: 2.39 MB

Other Supplementary Material for this manuscript includes the following:

  • PDB file S1. PDB coordinates of the best-scoring model.
  • PDB file S2. PDB coordinates of the largest cluster model.

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Citation: R. Mahajan, J. Ha, M. Zhang, T. Kawano, T. Kozasa, D. E. Logothetis, A Computational Model Predicts That Gβγ Acts at a Cleft Between Channel Subunits to Activate GIRK1 Channels. Sci. Signal. 6, ra69 (2013).

© 2013 American Association for the Advancement of Science