Supplementary Materials

Supplementary Materials for:

Conserved Regulators of Nucleolar Size Revealed by Global Phenotypic Analyses

Ralph A. Neumüller, Thomas Gross, Anastasia A. Samsonova, Arunachalam Vinayagam, Michael Buckner, Karen Founk, Yanhui Hu, Sara Sharifpoor, Adam P. Rosebrock, Brenda Andrews, Fred Winston,* Norbert Perrimon*

*Corresponding author. E-mail: perrimon@receptor.med.harvard.edu (N.P.); winston@genetics.med.harvard.edu (F.W.)

This PDF file includes:

  • Fig. S1. SGA screen for nucleolar defects in S. cerevisiae.
  • Fig. S2. Supporting graphs for nucleolar size quantification.
  • Fig. S3. The rDNA promoter proteome.
  • Fig. S4. Examples of protein complexes that scored in the SGA screen.
  • Fig. S5. A genome-wide RNAi screen for nucleolar size defects in Drosophila cell culture.
  • Fig. S6. Comparative GO term analysis between the S. cerevisiae SGA and the D. melanogaster RNAi screens.
  • Fig. S7. Altered nucleolar size upon loss of ribosomal function in D. melanogaster and S. cerevisiae.
  • Fig. S8. The Drosophila FACT complex is required for cell growth.
  • Fig. S9. The HIR complex double-mutant hir1Δhir2Δ shows increased Pol I occupancy over the 35S rDNA region.
  • Fig. S10. Growth measurements of HIR complex mutants.

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Technical Details

Format: Adobe Acrobat PDF

Size: 7.56 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). S. cerevisiae SGA screen.
  • Table S2 (Microsoft Excel format). GO term enrichment analyses (S. cerevisiae SGA screen).
  • Table S3 (Microsoft Excel format). Molecular complex analysis (S. cerevisiae).
  • Table S4 (Microsoft Excel format). TALO8 purification—35S rDNA–associated proteome in S. cerevisiae.
  • Table S5 (Microsoft Excel format). D. melanogaster dsRNAi screen.
  • Table S6 (Microsoft Excel format). Molecular complex analysis (D. melanogaster).
  • Table S7 (Microsoft Excel format). Evolutionarily conserved complexes between D. melanogaster and S.cerevisiae.
  • Table S8 (Microsoft Excel format). S. cerevisiae strains.
  • Table S9 (Microsoft Excel format). Primers.

[Download Tables S1 to S9]

  • 01 TALO8.RAW. Control plasmid for experiment 1.
  • Raw image files S1 to S4 of the TALO8 mass spectrometry data can be opened with ProteoWizard, which can be freely downloaded from http://proteowizard.sourceforge.net/. These files are in a ZIP archive.

    [Download Raw image files S1 to S4]


    Citation: R. A. Neumüller, T. Gross, A. A. Samsonova, A. Vinayagam, M. Buckner, K. Founk, Y. Hu, S. Sharifpoor, A. P. Rosebrock, B. Andrews, F. Winston, N. Perrimon, Conserved Regulators of Nucleolar Size Revealed by Global Phenotypic Analyses. Sci. Signal.6, ra70 (2013).

    © 2013 American Association for the Advancement of Science