Supplementary Materials

Supplementary Materials for:

Lipid-Induced Toxicity Stimulates Hepatocytes to Release Angiogenic Microparticles That Require Vanin-1 for Uptake by Endothelial Cells

Davide Povero, Akiko Eguchi, Ingrid R. Niesman, Nektaria Andronikou, Xavier de Mollerat du Jeu, Anny Mulya, Michael Berk, Milos Lazic, Samjana Thapaliya, Maurizio Parola, Hemal H. Patel, Ariel E. Feldstein*

*Corresponding author. E-mail: afeldstein@ucsd.edu

This PDF file includes:

  • Fig. S1. HepG2 exposed to different saturated and unsaturated FFAs.
  • Fig. S2. The effect of lipotoxic FFAs is counteracted by nonlipotoxic FFAs.
  • Fig. S3. Cellular localization and molecular function of proteins from hepatocyte-derived microparticles.
  • Fig. S4. Microparticles released by fat-laden rat primary hepatocytes are potent inducers of angiogenesis.
  • Fig. S5. The proangiogenic effect of hepatocyte-derived microparticles is dose-dependent.
  • Fig. S6. Hepatocyte-derived microparticles are detectable in HUVEC tube structures.
  • Fig. S7. Depletion of VNN1 reduces the ability of microparticles to promote HUVEC migration and tube formation.
  • Fig. S8. The proangiogenic effect of hepatocyte-derived microparticles requires VNN1-dependent internalization.
  • Fig. S9. The proangiogenic effects of VNN1-positive microparticles are not mediated by induction of cell proliferation or modulation of PPARγ expression.
  • Fig. S10. Increased mRNA expression of VNN1 in hepatocytes during lipotoxicity is independent of PPARα or PPARγ.
  • Fig. S11. Release of circulating microparticles depends on the stage of NASH.
  • Fig. S12. Circulating microparticles from mice with NASH stimulate endothelial cell function in vitro.
  • Fig. S13. Liver-specific silencing of VNN1 does not affect mRNA expression of VNN1 in other tissues.
  • Fig. S14. Mice treated with VNN1 siRNA showed reduced angiogenesis during diet-induced NASH.
  • Fig. S15. Casp3−/− mice show reduced MCD-induced pathological angiogenesis.
  • Table S1. Identification of proteins in hepatocyte-derived microparticles on the basis of the LC-MS/MS–derived sequences.

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Citation: D. Povero, A. Eguchi, I. R. Niesman, N. Andronikou, X. de Mollerat du Jeu, A. Mulya, M. Berk, M. Lazic, S. Thapaliya, M. Parola, H. H. Patel, A. E. Feldstein, Lipid-Induced Toxicity Stimulates Hepatocytes to Release Angiogenic Microparticles That Require Vanin-1 for Uptake by Endothelial Cells. Sci. Signal. 6, ra88 (2013).

© 2013 American Association for the Advancement of Science