Supplementary Materials

Supplementary Materials for:

Convergence of G Protein–Coupled Receptor and S-Nitrosylation Signaling Determines the Outcome to Cardiac Ischemic Injury

Z. Maggie Huang, Erhe Gao, Fabio Vasconcelos Fonseca, Hiroki Hayashi, Xiying Shang, Nicholas E. Hoffman, J. Kurt Chuprun, Xufan Tian, Doug G. Tilley, Muniswamy Madesh, David J. Lefer, Jonathan S. Stamler, Walter J. Koch*

*Corresponding author. E-mail: walter.koch@temple.edu

This PDF file includes:

  • Fig. S1. Ischemia/reperfusion injury in GRK2 Tg or βARKct Tg mice in which eNOS abundance was genetically manipulated.
  • Fig. S2. Generation of GRK2-C340S knock-in mouse and real-time PCR and Western blot measurements of the abundance of βAR signaling pathway components in wild-type and GRK2-C340S knock-in mouse hearts.
  • Fig. S3. Echocardiography measurements in wild-type and GRK2-C340S mice.
  • Fig. S4. Biochemical characteristics of GRK2 in wild-type and GRK2-C340S knock-in mouse hearts.
  • Fig. S5. Decline in the in vivo LV contractility of wild-type and GRK2-C340S mice during isoproterenol infusion.
  • Fig. S6. Characteristics of ischemia/reperfusion injury in wild-type and GRK2-C340S knock-in mice after GSNO treatment.
  • Fig. S7. Characteristics of ischemia/reperfusion injury in eNOS/GRK2-C340S knock-in mice and wild-type mice acutely infused with GSNO.
  • Fig. S8. Activation of the mitogen-activated protein kinase p42/p44 ERK in NRVMs treated with isoproterenol or isoproterenol and EGFR inhibitor.

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Citation: Z. M. Huang, E. Gao, F. V. Fonseca, H. Hayashi, X. Shang, N. E. Hoffman, J. K. Chuprun, X. Tian, D. G. Tilley, M. Madesh, D. J. Lefer, J. S. Stamler, W. J. Koch, Convergence of G Protein–Coupled Receptor and S-Nitrosylation Signaling Determines the Outcome to Cardiac Ischemic Injury. Sci. Signal. 6, ra95 (2013).

© 2013 American Association for the Advancement of Science