Supplementary Materials

Supplementary Materials for:

Protein Interaction Network of the Mammalian Hippo Pathway Reveals Mechanisms of Kinase-Phosphatase Interactions

Amber L. Couzens, James D. R. Knight, Michelle J. Kean, Guoci Teo, Alexander Weiss, Wade H. Dunham, Zhen-Yuan Lin, Richard D. Bagshaw, Frank Sicheri, Tony Pawson, Jeffrey L. Wrana, Hyungwon Choi, Anne-Claude Gingras*

*Corresponding author. E-mail:

This PDF file includes:

  • Fig. S1. Bait protein expression in inducible cell lines.
  • Fig. S2. High-resolution heatmap for the FLAG AP-MS data. Fig. S3. High-resolution heatmap for the BioID data.
  • Fig. S4. Network diagram depicting the complete Hippo interactome.
  • Fig. S5. Detailed Lats2 interactome.
  • Fig. S6. Detailed LATS1 interactome.
  • Fig. S7. Comparison of Lats2 FLAG AP-MS and BioID data sets.
  • Fig. S8. Detailed YAP1 interactome.
  • Fig. S9. Detailed MST1 interactome.
  • Fig. S10. Detailed MST2 interactome.
  • Fig. S11. Detailed YWHAB interactome.
  • Fig. S12. Detailed MOB1A interactome.
  • Fig. S13. Detailed MOB1B interactome.
  • Fig. S14. Model for MST1 and MST2 recruitment to STRIPAK.
  • Fig. S15. Validation of specificity of PPP6C antibody.

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Technical Details

Format: Adobe Acrobat PDF

Size: 5.54 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Proteins used as baits.
  • Table S2 (Microsoft Excel format). Composition of the FLAG AP-MS data set.
  • Table S3 (Microsoft Excel format). Composition of the in vivo biotinylation data set.
  • Table S4 (Microsoft Excel format). Composition of the additional FLAG AP-MS data set.
  • Table S5 (Microsoft Excel format). High-confidence data set reported in this study.
  • Table S6 (Microsoft Excel format). Time course of OA treatment.
  • Table S7 (Microsoft Excel format). Comparison of the MOB-family protein interactions.
  • Table S8 (Microsoft Excel format). List of antibodies used in this study.
  • Website_tutorial.pdf. Tutorial to the Web site.
  • Hippo_interactome.cys. Cytoscape file depicting the complete Hippo interactome.

[Download Tables S1 to S8]

[Download Website_tutorial]

[Download Hippo_interactome]

Citation: A. L. Couzens, J.D.R.Knight,M. J.Kean, G. Teo, A.Weiss,W. H. Dunham, Z.-Y. Lin, R. D. Bagshaw, F. Sicheri, T. Pawson, J. L. Wrana, H. Choi, A.-C. Gingras, Protein interaction network of the mammalian Hippo pathway reveals mechanisms of kinase-phosphatase interactions. Sci. Signal. 6, rs15 (2013).

© 2013 American Association for the Advancement of Science