Supplementary Materials

Supplementary Materials for:

The ζ Isoform of Diacylglycerol Kinase Plays a Predominant Role in Regulatory T Cell Development and TCR-Mediated Ras Signaling

Rohan P. Joshi, Amanda M. Schmidt, Jayajit Das, Dariusz Pytel, Matthew J. Riese, Melissa Lester, J. Alan Diehl, Edward M. Behrens, Taku Kambayashi, Gary A. Koretzky*

*Corresponding author. E-mail: koretzky@mail.med.upenn.edu

This PDF file includes:

  • Fig. S1. Wild-type, DGKα-deficient, and DGKζ-deficient mice have similar percentages of CD4 SP cells.
  • Fig. S2. Phosphorylation of IkBa is increased similarly in DGKα- and DGKζ- deficient T cells in response to TCR stimulation.
  • Fig. S3. ERK phosphorylation after TCR stimulation is independent of the cell surface abundance of CD44 on CD8+ T cells.
  • Fig. S4. MEK inhibition abrogates ERK phosphorylation after TCR engagement in wild-type, DGKα-deficient, and DGKζ-deficient T cells.
  • Fig. S5. The abundance of DGKα is greater than that of DGKζ in T cells.
  • Fig. S6. DGKα is overexpressed in cells transduced with a retrovirus encoding a nontagged form of DGKα.
  • Fig. S7. DGKα and DGKζ are diffusely localized in unconjugated T cells.
  • Fig. S8. DGKα and DGKζ localize to the T cell–APC contact site.
  • Fig. S9. DGKα and DGKζ localize similarly at the T cell–APC contact site and at the immunological synapse, as defined by talin.
  • Fig. S10. In silico signaling model.
  • Fig. S11. The ability of DGKζ to suppress signaling downstream of Ras depends on its kinase activity.
  • Table S1. Reactions and the kinetic rates used in the in silico model.
  • Table S2. Concentrations of the proteins and lipids used in the in silico model.
  • Reference (44)

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Citation: R. P. Joshi, A. M. Schmidt, J. Das, D. Pytel, M. J. Riese, M. Lester, J. A. Diehl, E. M. Behrens, T. Kambayashi, G. A. Koretzky, The ζ Isoform of Diacylglycerol Kinase Plays a Predominant Role in regulatory T cell development and TCR-mediated Ras Signaling. Sci. Signal. 6, ra102 (2013).

© 2013 American Association for the Advancement of Science