Supplementary Materials

Supplementary Materials for:

Noncanonical NF-κB Signaling Is Limited by Classical NF-κB Activity

Carolyn M. Gray, Caroline Remouchamps, Kelly A. McCorkell, Laura A. Solt, Emmanuel Dejardin, Jordan S. Orange, Michael J. May*

*Corresponding author. E-mail:

This PDF file includes:

  • Fig. S1. Basal and anti-LTβR–induced p100 processing in IKKα- and NEMO-deficient cells.
  • Fig. S2. Quantification of nuclear p52 and RelB amounts and analysis of gene expression in IKK-deficient MEFs.
  • Fig. S3. Wild-type NEMO rescues basal p100 processing in NEMOKO cells.
  • Fig. S4. Classical NF-κB signaling in NEMO-reconstituted MEFs.
  • Fig. S5. Generation of IKKαWT and IKKαΔNBD MEF cell lines.
  • Fig. S6. Increased NIK abundance in IKKβ-deficient MEF cell lines.
  • Fig. S7. Classical NF-κB activity in reconstituted IKKβKO MEFs.
  • Fig. S8. Increased NIK abundance in IKKα/β DKO MEFs.
  • Fig. S9. Expression of birc3 and components of the basal NIK regulatory complex in various MEF cell lines.
  • Fig. S10. FLAG-NIK associates with the endogenous basal NIK regulatory complex in NEMOKO and p65KO MEFs.

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Citation: C. M. Gray, C. Remouchamps, K. A. McCorkell, L. A. Solt, E. Dejardin, J. S. Orange, M. J. May, Noncanonical NF-κB Signaling Is Limited by Classical NF-κB Activity. Sci. Signal. 7, ra13 (2014).

© 2014 American Association for the Advancement of Science