Supplementary Materials

Supplementary Materials for:

Distinct Cytoplasmic Domains in Plexin-A4 Mediate Diverse Responses to Semaphorin 3A in Developing Mammalian Neurons

Guy Mlechkovich, Sheng-Shiang Peng, Vered Shacham, Edward Martinez, Irena Gokhman, Adi Minis, Tracy S. Tran,* Avraham Yaron*

*Corresponding author. E-mail: avraham.yaron@weizmann.ac.il (A.Y.); tstran@rutgers.edu (T.S.T.)

This PDF file includes:

  • Fig. S1. Overexpression of Plexin-A4 does not enhance growth cone collapse in response to Sema3A.
  • Fig. S2. Plexin-A4 receptor variants are expressed on cell surface.
  • Fig. S3. Cell surface detection of Plexin-A4 variants on cortical neuron soma and neurites.
  • Fig. S4. Plexin-A4 cytoplasmic domain variants regulate dendritic morphogenesis of wild-type and PlxnA4−/− cortical neurons.
  • Fig. S5. Axonal expression of Plexin-A4 variants in cortical neurons.
  • Fig. S6. Sema3A-induced Plexin-A4 signaling does not alter total axonal length in wild-type cortical neurons.
  • Fig. S7. Specific Plexin-A4 cytoplasmic domains regulate cellular collapse in COS7 cells.
  • Fig. S8. Interaction of Plexin-A4 active and nonactive variants with neuropilin-1.
  • Fig. S9. Expression of FARP1 and FARP2 in the mouse cortex and DRG.
  • Fig. S10. Western blots illustrate FARP protein knockdown by specific siRNAs in primary cortical and DRG neuron cultures.
  • Reference (57)

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Citation: G. Mlechkovich, S.-S. Peng, V. Shacham, E. Martinez, I. Gokhman, A. Minis, T. S. Tran, A. Yaron, Distinct Cytoplasmic Domains in Plexin-A4 Mediate Diverse Responses to Semaphorin 3A in Developing Mammalian Neurons. Sci. Signal. 7, ra24 (2014).

© 2014 American Association for the Advancement of Science