Supplementary Materials

Supplementary Materials for:

MeCP2 Reinforces STAT3 Signaling and the Generation of Effector CD4+ T Cells by Promoting miR-124–Mediated Suppression of SOCS5

Shan Jiang, Chaoran Li, Gabrielle McRae, Erik Lykken, Jose Sevilla, Si-Qi Liu, Ying Wan,* Qi-Jing Li*

*Corresponding author. E-mail: Qi-Jing.Li@Duke.edu (Q.-J.L.); wanying.cn@gmail.com (Y.W.)

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  • Fig. S1. CD4+ T cell development is normal in mice with T cell–specific deletion of Mecp2.
  • Fig. S2. T cells in Mecp2-deficient mice do not undergo spontaneous activation.
  • Fig. S3. Loss of MeCP2 does not alter antigen-dependent proliferation or activation-induced cell death.
  • Fig. S4. Chromatin accessibility of the Il17 and Ifng loci in naïve CD4+CD25 T cells.
  • Fig. S5. Abundances of mRNAs for components of STAT signaling pathways.
  • Fig. S6. The dynamics of SOCS5 abundance during the activation of CD4+ T cells in the presence of IL-6.
  • Fig. S7. SOCS5 inhibits the activation of STAT1 and the differentiation of naïve CD4+ T cells.
  • Fig. S8. The chromatin accessibility of the Socs5 locus in naïve CD4+CD25 T cells.
  • Fig. S9. miR-124 was computationally predicted to target Socs5 mRNA at a highly conserved site.
  • Fig. S10. Activated MeCP2-deficient CD4+ T cells have decreased amounts of pri-miR-124 and mature miR-124 compared to those of wild-type CD4+ cells.
  • Fig. S11. Retroviral-mediated overexpression of miR-124 in 3T3 cells and CD4+ T cells.
  • Fig. S12. miR-124 promotes IL-6–induced STAT3 activation in CD4+ T cells.
  • Fig. S13.The MeCP2–miR-124–SOCS5 axis enables CD4+ T cell differentiation.
  • Fig. S14. Loss of a single copy of Mecp2 does not adversely affect the generation of TH1 and TH17 mouse T cells.

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Citation: S. Jiang, C. Li, G. McRae, E. Lykken, J. Sevilla, S.-Q. Liu, Y. Wan, Q.-J. Li, MeCP2 Reinforces STAT3 Signaling and the Generation of Effector CD4+ T Cells by Promoting miR-124–Mediated Suppression of SOCS5. Sci. Signal. 7, ra25 (2014).

© 2014 American Association for the Advancement of Science