Supplementary Materials

Supplementary Materials for:

Huwe1-Mediated Ubiquitylation of Dishevelled Defines a Negative Feedback Loop in the Wnt Signaling Pathway

Reinoud E. A. de Groot, Ranjani S. Ganji, Ondrej Bernatik, Bethan Lloyd-Lewis, Katja Seipel, Kateřina Šedová, Zbynĕk Zdráhal, Vishnu M. Dhople, Trevor C. Dale, Hendrik C. Korswagen,* Vitezslav Bryja*

*Corresponding author. E-mail: r.korswagen@hubrecht.eu (H.C.K.); bryja@sci.muni.cz (V.B.)

This PDF file includes:

  • Fig. S1. eel-1 is a negative regulator of Wnt signaling in C. elegans.
  • Fig. S2. eel-1/Huwe1 is a negative regulator of Wnt signaling that acts upstream of β-catenin in the Wnt pathway.
  • Fig. S3. Huwe1 interacts with Dvl and promotes Dvl ubiquitylation.
  • Fig. S4. Huwe1 promotes Dvl ubiquitylation.
  • Fig. S5. Huwe1 inhibits Dvl multimerization.
  • Table S1. List of DUBs, E2 enzymes, and E3 ubiquitin ligases that were screened for enhancement or suppression of the vps-29(tm1320)–induced QL.d migration phenotype.
  • Table S2. Effect of eel-1(RNAi) on the EGL-20/Wnt–dependent migration of the QL.d in various Wnt pathway mutants.
  • Legends for tables S3 and S4
  • Table S5. MS analysis of ubiquitin chain linkage ligated to Dvl1.

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Technical Details

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Other Supplementary Material for this manuscript includes the following:

  • Table S3 (Microsoft Excel format). MS analysis of binding partners of Dvl immunoprecipitated from MEFs.
  • Table S4 (Microsoft Excel format). MS analysis of ubiquitylated lysine residues of Dvl1.

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Citation: R. E. A. de Groot, R. S. Ganji, O. Bernatik, B. Lloyd-Lewis, K. Seipel, K. Šedová, Z. Zdráhal, V. M. Dhople, T. C. Dale, H. C. Korswagen, V. Bryja, Huwe1-Mediated Ubiquitylation of Dishevelled Defines a Negative Feedback Loop in the Wnt Signaling Pathway. Sci. Signal. 7, ra26 (2014).

© 2014 American Association for the Advancement of Science