Supplementary Materials

Supplementary Materials for:

Arginine Starvation Impairs Mitochondrial Respiratory Function in ASS1-Deficient Breast Cancer Cells

Fuming Qiu, Yun-Ru Chen, Xiyong Liu, Cheng-Ying Chu, Li-Jiuan Shen, Jinghong Xu, Shikha Gaur, Henry Jay Forman, Hang Zhang, Shu Zheng, Yun Yen, Jian Huang, Hsing-Jien Kung, David K. Ann*

*Corresponding author. E-mail: dann@coh.org

This PDF file includes:

  • Fig. S1. Knockdown of ASS1 increases sensitivity to ADI-PEG20 treatment.
  • Fig. S2. Autophagy is required for ADI-PEG20–induced cell death.
  • Fig. S3. Arginine depletion suppresses the basal oxygen consumption rate and reserve capacity.
  • Fig. S4. ADI-PEG20 does not affect SOD and SIRT mRNA abundance, but decreases mitochondrial protein abundance.
  • Fig. S5. ADI-PEG20 causes mitochondrial fragmentation in MDA-MB-231 cells.
  • Fig. S6. ASS1 abundance predicts OS.
  • Fig. S7. Lack of synergistic effect of ADI-PEG20 combined with Abraxane or carboplatin.
  • Table S1. Primer sequences.

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Citation: F. Qiu, Y. -R. Chen, X. Liu, C. -Y. Chu, L. -J. Shen, J. Xu, S. Gaur, H. J. Forman, H. Zhang, S. Zheng, Y. Yen, J. Huang, H.-J. Kung, D. K. Ann, Arginine Starvation Impairs Mitochondrial Respiratory Function in ASS1-Deficient Breast Cancer Cells. Sci. Signal. 7, ra31 (2014).

© 2014 American Association for the Advancement of Science