Supplementary Materials

Supplementary Materials for:

Specificity of the CheR2 Methyltransferase in Pseudomonas aeruginosa Is Directed by a C-Terminal Pentapeptide in the McpB Chemoreceptor

Cristina García-Fontana, Andrés Corral Lugo, Tino Krell*

*Corresponding author. E-mail: tino.krell@eez.csic.es

This PDF file includes:

  • Fig. S1. Microcalorimetric titrations of the four CheR paralogs of P. aeruginosa PAO1 with S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH).
  • Fig. S2. Microcalorimetric titration of CheR2 with the NWETF pentapeptide.
  • Fig. S3. Lack of an allosteric interaction between the pentapeptide-binding site and the SAM/SAH-binding site in CheR2.
  • Fig. S4. Sequence alignment of different CheR sequences.
  • Fig. S5. SAH binding by CheR2ΔGPN.
  • Fig. S6. Clustering of CheR sequences after deletion of the GXX tripeptide.
  • Fig. S7. Microcalorimetric titrations of CheR1+GPN with the GWEEF pentapeptide or SAH.
  • Table S1. Lack of allostery between the pentapeptide-binding site and the SAM/SAH-binding site in CheR2.
  • Table S2. Strains and plasmids used in this study.
  • Table S3. Oligonucleotides used in this study.
  • References (4446)

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Citation: C. García-Fontana, A. Corral Lugo, T. Krell, Specificity of the CheR2Methyltransferase in Pseudomonas aeruginosa Is Directed by a C-Terminal Pentapeptide in the McpB Chemoreceptor. Sci. Signal. 7, ra34 (2014).

© 2014 American Association for the Advancement of Science