Supplementary Materials

Supplementary Materials for:

Chloride Sensing by WNK1 Involves Inhibition of Autophosphorylation

Alexander T. Piala, Thomas M. Moon, Radha Akella, Haixia He, Melanie H. Cobb, Elizabeth J. Goldsmith*

*Corresponding author. E-mail: elizabeth.goldsmith@utsouthwestern.edu

This PDF file includes:

  • Fig. S1. Raw trace of WNK1-KDm/S* DSF using SYPRO Orange.
  • Fig. S2. Control kinase stability as a function of salt concentration.
  • Fig. S3. Phosphorylation of OSR1 by WNK1-KDm/S*.
  • Fig. S4. WNK1 autophosphorylation measured by mass spectrometry.
  • Fig. S5. Interaction of bound chloride with hydrophobic residues.
  • Table S1. Data and refinement statistics for WNK1-sKDm/S*.
  • Table S2. B-factors of the 3/10 helix residues in WNK1-KDm/SA and WNK1- sKDm/S*.
  • Table S3. Phosphorylation state of WNK1-KDm/S* mutants after purification from E. coli.

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Citation: A. T. Piala, T. M. Moon, R. Akella, H. He, M. H. Cobb, E. J. Goldsmith, Chloride Sensing by WNK1 Involves Inhibition of Autophosphorylation. Sci. Signal. 7, ra41 (2014).

© 2014 American Association for the Advancement of Science