Supplementary Materials

Supplementary Materials for:

Downstream of Mutant KRAS, the Transcription Regulator YAP Is Essential for Neoplastic Progression to Pancreatic Ductal Adenocarcinoma

Weiying Zhang, Nivedita Nandakumar, Yuhao Shi, Mark Manzano, Alias Smith, Garrett Graham, Swati Gupta, Eveline E. Vietsch, Sean Z. Laughlin, Mandheer Wadhwa, Mahandranauth Chetram, Mrinmayi Joshi, Fen Wang, Bhaskar Kallakury, Jeffrey Toretsky, Anton Wellstein, Chunling Yi*

*Corresponding author. E-mail: cy232@georgetown.edu

This PDF file includes:

  • Fig. S1. YAP is expressed in PDAC.
  • Fig. S2. Deletion of Yap does not affect normal pancreatic development and glucose metabolism.
  • Fig. S3. Ablation of Yap blocks PDAC development in Kras and Kras:Trp53 mutant pancreas.
  • Fig. S4. Yap is not required for oncogenic Kras–induced ADM in vitro.
  • Fig. S5. Yap is not required for induction of ADM by acute pancreatitis in vivo.
  • Fig. S6. Deletion of Yap does not cause significant changes in apoptosis or senescence.
  • Fig. S7. Characterization of Yap+ and Yap primary Kras:Trp53 mutant pancreatic ductal cells.
  • Fig. S8. Yap selectively promotes the expression of a group of genes encoding oncogenic Kras–induced secretory factors.
  • Fig. S9. Ser367 is the primary phosphorylation site responsible for the oncogenic RAS–induced mobility shift of YAP.
  • Table S1. Abbreviations of all the mouse genotypes used in this study.
  • Table S2. IHC analysis of YAP in human pancreatic TMAs.
  • Table S3. Mouse and human primers used in qRT-PCR assay.
  • Table S4. Mouse primers used in ChIP assay.
  • Table S5. Antibodies used in IHC and Western blot analyses.

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Citation: W. Zhang, N. Nandakumar, Y. Shi, M. Manzano, A. Smith, G. Graham, S. Gupta, E. E. Vietsch, S. Z. Laughlin, M. Wadhwa, M. Chetram, M. Joshi, F. Wang, B. Kallakury, J. Toretsky, A. Wellstein, C. Yi, Downstream of Mutant KRAS, the Transcription Regulator YAP Is Essential for Neoplastic Progression to Pancreatic Ductal Adenocarcinoma. Sci. Signal. 7, ra42 (2014).

© 2014 American Association for the Advancement of Science