Supplementary Materials

Supplementary Materials for:

A Nanoparticle-Based Combination Chemotherapy Delivery System for Enhanced Tumor Killing by Dynamic Rewiring of Signaling Pathways

Stephen W. Morton, Michael J. Lee, Zhou J. Deng, Erik C. Dreaden, Elise Siouve, Kevin E. Shopsowitz, Nisarg J. Shah, Michael B. Yaffe,* Paula T. Hammond*

*Corresponding author. E-mail: myaffe{at}mit.edu (M.B.Y.); hammond{at}mit.edu (P.T.H.)

This PDF file includes:

  • Fig. S1. Release of doxorubicin visualized by confocal microscopy.
  • Fig. S2. Biodistribution of Cy5.5-labeled empty liposomes (lacking drug) intravenously administered to mice bearing BT-20 or A549 xenografts.
  • Fig. S3. Tumor growth monitored by bioluminescence of the firefly luciferase.
  • Fig. S4. BT-20 tumor bioluminescence in untreated mice, mice treated with singledrug liposomes, or mice treated with dual-drug liposomes.
  • Fig. S5. A549 tumor bioluminescence in untreated mice, mice treated with singledrug liposomes, or mice treated with dual-drug liposomes.
  • Fig. S6. Effect of a coadministration paradigm on A549 xenograft growth.
  • Fig. S7. Effect of pH on the in vitro release of drugs from liposomes containing different combinations of RTK inhibitor and doxorubicin.
  • Fig. S8. Abundance of cleaved caspase-8, pERK, and γH2AX in A549 or BT-20 cells exposed to different dual drug–loaded folate-functionalized liposomes.
  • Table S1. Characterization of nonfunctionalized (without folate-PEG) dual drug–loaded and single drug–loaded liposomes.

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Citation: S. W. Morton, M. J. Lee, Z. J. Deng, E. C. Dreaden, E. Siouve, K. E. Shopsowitz, N. J. Shah, M. B. Yaffe, P. T. Hammonda, A Nanoparticle-Based Combination Chemotherapy Delivery System for Enhanced Tumor Killing by Dynamic Rewiring of Signaling Pathways. Sci. Signal. 7, ra44 (2014).

© 2014 American Association for the Advancement of Science