Supplementary Materials

Supplementary Materials for:

Reconstituted Human TPC1 Is a Proton-Permeable Ion Channel and Is Activated by NAADP or Ca2+

Samantha J. Pitt, Andy K. M. Lam, Katja Rietdorf, Antony Galione, Rebecca Sitsapesan*

*Corresponding author. E-mail: rebecca.sitsapesan{at}pharm.ox.ac.uk

This PDF file includes:

  • Fig. S1. The response of mock affinity-purified preparations to NAADP.
  • Fig. S2. Effect of luminal NAADP on TPC1 gating.
  • Fig. S3. TPC1 relative permeability of K+, Na+, and Ca2+ to H+.
  • Fig. S4. Effect of PI(3,5)P2 on TPC1 gating in the presence of activating concentrations of Ca2+ on the cytosolic side.

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Citation: S. J. Pitt, A. K. M. Lam, K. Rietdorf, A. Galione, R. Sitsapesan, Reconstituted Human TPC1 Is a Proton-Permeable Ion Channel and Is Activated by NAADP or Ca2+. Sci. Signal. 7, ra46 (2014).

© 2014 American Association for the Advancement of Science