Supplementary Materials

Supplementary Materials for:

A PLCγ1-Dependent, Force-Sensitive Signaling Network in the Myogenic Constriction of Cerebral Arteries

Albert L. Gonzales, Ying Yang, Michelle N. Sullivan, Lindsey Sanders, Fabrice Dabertrand, David C. Hill-Eubanks, Mark T. Nelson, Scott Earley*

*Corresponding author. E-mail: searley@medicine.nevada.edu

This PDF file includes:

  • Fig. S1. PLC activity is necessary for stretch-induced TRPM4 activity in arterial myocytes.
  • Fig. S2. Control experiments for U73122.
  • Fig. S3. PLC activity is necessary for myogenic and agonist-induced constriction.
  • Fig. S4. Control experiments for siRNA-mediated PLCγ1 down-regulation.
  • Fig. S5. Rate of Ca2+ depletion within the SR in freshly isolated smooth muscle cells.
  • Fig. S6. GsMTx-4 and a TRPC6 inhibitory antibody do not block TRPM4 channels.
  • Fig. S7. Src kinase inhibitor-1 blocks stretch-dependent TICC activity and myogenic tone.
  • Fig. S8. Activation of TRPC6 with OAG (1-oleoyl-2-acetyl-sn-glycerol).

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Citation: A. L. Gonzales,Y. Yang, M. N.Sullivan, L. Sanders, F. Dabertrand, D. C. Hill-Eubanks, M. T. Nelson, S. Earley, A PLCγ1-Dependent, Force-Sensitive Signaling Network in the Myogenic Constriction of Cerebral Arteries. Sci. Signal. 7, ra49 (2014).

© 2014 American Association for the Advancement of Science