Supplementary Materials

Supplementary Materials for:

Multifaceted Activities of Type I Interferon Are Revealed by a Receptor Antagonist

Doron Levin, William M. Schneider, Hans-Heinrich Hoffmann, Ganit Yarden, Alberto Giovanni Busetto, Ohad Manor, Nanaocha Sharma, Charles M. Rice, Gideon Schreiber*

*Corresponding author. E-mail:

This PDF file includes:

  • Fig. S1. IFN-1ant does not cause a change in cell surface receptor abundance.
  • Fig. S2. The effect of increasing the concentration of IFN-1ant on the antiproliferative activity of IFN-α2.
  • Fig. S3. Increasing the concentration of IFN-α2 to 1 nM restores its antiviral activity.
  • Fig. S4. T47D cells are resistant to EMCV infection.
  • Fig. S5. IFN-1ant and IFNL3 have additive antiviral effects.
  • Fig. S6. Increased concentrations of IFN-α2 lead to increased amounts of transcripts.
  • Fig. S7. Treatment of IFNAR1 knockout cells with IFN does not induce gene expression.
  • Fig. S8. Comparison between standard qPCR analysis and the BioMark real-time PCR system.
  • Fig. S9. The information processing flow for the classification and clustering of genes.
  • Fig. S10. Inhibition of viral replication occurs at concentrations of IFN that do not inhibit cell proliferation.
  • Table S1. Categorization of genes according to the classification analysis based on gene expression profiles.
  • Table S2. List of genes used for promoter analysis of transcription factor binding sites.
  • Table S3. Complete list of genes used in the BioMark real-time PCR analysis.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 530 KB

Citation: D. Levin, W. M. Schneider, H.-H. Hoffmann, G. Yarden, A. G. Busetto, O. Manor, N. Sharma, C. M. Rice, G. Schreiber, Multifaceted Activities of Type I Interferon Are Revealed by a Receptor Antagonist. Sci. Signal. 7, ra50 (2014).

© 2014 American Association for the Advancement of Science