Supplementary Materials

Supplementary Materials for:

Interplay Between the Oxidoreductase PDIA6 and microRNA-322 Controls the Response to Disrupted Endoplasmic Reticulum Calcium Homeostasis

Jody Groenendyk, Zhenling Peng, Elzbieta Dudek, Xiao Fan, Marcin J. Mizianty, Estefanie Dufey, Hery Urra, Denisse Sepulveda, Diego Rojas-Rivera, Yunki Lim, Do Han Kim, Kayla Baretta, Sonal Srikanth, Yousang Gwack, Joohong Ahnn, Randal J. Kaufman, Sun-Kyung Lee, Claudio Hetz, Lukasz Kurgan, Marek Michalak*

*Corresponding author. E-mail: marek.michalak@ualberta.ca

This PDF file includes:

  • Fig. S1. The XBP1 splicing reporter system.
  • Fig. S2. A genome-wide siRNA screen.
  • Fig. S3. Silencing of the PDIA6 gene.
  • Fig. S4. Phosphorylation of eIF2α in PDIA6-silenced cells.
  • Fig. S5. PDIA6 mRNA abundance under ER stress conditions.
  • Fig. S6. PDIA6 interacts with the luminal domain of IRE1.
  • Fig. S7. PDIA6 silencing and IRE1α RIDD activity.
  • Fig. S8. Selected miRNAs identified by deep sequencing analysis.
  • Fig. S9. Schematic representation of multiple target prediction analysis.

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Citation: J. Groenendyk, Z. Peng, E. Dudek, X. Fan, M. J. Mizianty, E. Dufey, H. Urra, D. Sepulveda, D. Rojas-Rivera, Y. Lim, D. H. Kim, K. Baretta, S. Srikanth, Y. Gwack, J. Ahnn, R. J. Kaufman, S.-K. Lee, C. Hetz, L. Kurgan, M. Michalak, Interplay Between the Oxidoreductase PDIA6 and microRNA-322 Controls the Response to Disrupted Endoplasmic Reticulum Calcium Homeostasis. Sci. Signal. 7, ra54 (2014).

© 2014 American Association for the Advancement of Science