Supplementary Materials

Supplementary Materials for:

Memo Is a Copper-Dependent Redox Protein with an Essential Role in Migration and Metastasis

Gwen MacDonald, Ivan Nalvarte, Tatiana Smirnova, Manuela Vecchi, Nicola Aceto, Arno Doelemeyer, Anna Frei, Susanne Lienhard, Jeffrey Wyckoff, Daniel Hess, Jan Seebacher, Jeremy J. Keusch, Heinz Gut, Daniele Salaun, Giovanni Mazzarol, Davide Disalvatore, Mohamed Bentires-Alj, Pier Paolo Di Fiore, Ali Badache, Nancy E. Hynes*

*Corresponding author. E-mail:

This PDF file includes:

  • Fig. S1. Memo is required for cellular migration and invasion; Memo overexpression is not transforming.
  • Fig. S2. Memo down-regulation does not decrease in vitro cellular proliferation or primary tumor outgrowth, but may decrease the survival or proliferation of disseminated tumor cells in the lungs.
  • Fig. S3. Reconstitution of Memo restores invasion and tumor cell migration in vivo.
  • Fig. S4. Analyses of Memo activity.
  • Fig. S5. Memo influences the intracellular redox status.
  • Fig. S6. Analysis of ROS and selected mRNAs in Memo knockdown cell lines.
  • Fig. S7. Memo immunohistochemistry.
  • Table S1. Clinicopathological characteristics of the breast cancer cohort analyzed for Memo abundance.
  • Table S2. Correlations between Memo status and clinical and pathological parameters in the breast cancer cohort.
  • Table S3. Observed events in the breast cancer cohort.
  • Table S4. A time-dependent multivariable Cox model to estimate HRs.
  • Table S5. Primers, oligos, and vectors used.
  • Legends for movies S1 to S3

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 601 KB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Motility of MDA-MB-231 tumor cells expressing shLZ.
  • Movie S2 (.mov format). Cell protrusions in MDA-MB-231 tumor cells with sh5- mediated Memo knockdown.
  • Movie S3 (.avi format). Motility of sh5-expressing MDA-MB-231 tumor cells reconstituted with Memo.

[Download Movies S1 to S3]

Citation: G. MacDonald, I. Nalvarte, T. Smirnova, M. Vecchi, N. Aceto, A. Dolemeyer, A. Frei, S. Lienhard, J. Wyckoff, D. Hess, J. Seebacher, J. J. Keusch, H. Gut, D. Salaun, G. Mazzarol, D. Disalvatore, M. Bentires-Alj, P. P. Di Fiore, A. Badache, N. E. Hynes, Memo Is a Copper-Dependent Redox Protein with an Essential Role in Migration and Metastasis. Sci. Signal. 7, ra56 (2014).

© 2014 American Association for the Advancement of Science